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Phosphate buffer (100 mM K 2 HPO 4 /KH 2 PO 4 , 1 mM EDTA, pH 7.4) with 1 M of the respective roGFP according to the information given in the text were pipetted into the wells of a 96-well plate with a clear bottom (NUNC 96, www.thermoscientific.com)
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A urine or plasma amino acid profile can identify excess ammonia as well as levels of homocysteine, glutathione, cysteine, and taurine
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This is why a deficiency in these nutrients is linked to digestive challenges
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