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This method was introduced to reduce a bias in which firms who offer health benefits face a longer average survey than non-offering firms

Rotate sites systematically (abdomen at least 2 inches from navel, thighs, upper arms, flank) to avoid lipohypertrophy [7]

The biofilm suspension was then serially diluted in 1 PBS and 100 ml aliquots were spread on TSB plates and incubated for 24 h at 37C

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