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Mir-135 suppresses glycolysis and promotes pancreatic cancer cell adaptation to metabolic stress by targeting phosphofructokinase-1

References Hajihashemi P, Askari G, Khorvash F, Reza Maracy M, Nourian M

Liver tissue was crushed and prepared with previously described methods to separate the aqueous, lipid, and protein layers, 9 and sent to the Department of Biochemistry at the University of Cambridge, Cambridge, UK, where liquid chromatography mass spectrometry was undertaken

Third-party verification eliminates conflict of interest in quality assessment and provides researchers with independent confirmation of compound specifications

We used a modified Cochrane risk of bias tool to assess the risk of bias, used GRADE to assess the certainty of evidence, and used the Credibility of Effect Modification Analyses (ICEMAN) tool to assess the credibility of any apparent subgroup effect