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Aggregation occurs when peptides are exposed to mechanical agitation (shaking), rapid temperature changes (freeze-thaw cycles), or prolonged storage above 8C

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Key Takeaway: The kidneys are high-energy organs that produce significant oxidative waste

Conversely, this auto-oxidation, which is detrimental to the nitrosothiol formation, contributes to the production of highly reactive peroxynitrite resulting from the reaction between superoxide anions and NO