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The effect of sleeve gastrectomy on GLP-1 secretion and gastric emptying: a prospective study

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As insulin resistance develops, the liver becomes less sensitive to insulin, which decreases glucose uptake and increases glucose production by the liver, which in turn worsens hyperglycemia (118)

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The samples were incubated on ice and centrifuged to separate the serum, which was then collected, transferred to clean tubes, and stored until further experimentation took place [2]