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Fifty microliters of stop solution was added to each well and the optical density at 450 nm determined using a microtiter plate reader (Liuyi, Beijing, China) within 15 min

The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the primary antibody (ET1702-09) at 1/1,000 dilution for 1 hour at room temperature

Tablets are another popular format, often combined with supportive nutrients like vitamin C or alpha lipoic acid

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