Phenotypic screening is the pragmatic alternative: apply the peptide to a relevant cell system and measure broad outputs, transcriptome by RNA-seq, proteome, or metabolic and viability panels, without presupposing a target
Thats not an abstract regulatory complaintit directly affects safety and predictability
Then you catch your reflection in the bathroom mirror under harsh lighting and reality hits - your face didn't get the memo
Wang CH, Tsuji T, Wu LH, Yang CY, Huang TL, Sato M, et al
Protein turnover releases - N -trimethyllysine, which is hydroxylated by - N -trimethyllysine hydroxylase in a reaction requiring Fe 2+ and ascorbate, producing -hydroxy-- N -trimethyllysine