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(2025, August 15)
Glutathione uptake assay in immunopurified mitochondria Mitochondria were purified from HEK-293T cells expressing 3xHA-OMP25-mCherry (mitochondrial isolation) or 3xMyc-OMP25-mCherry (background control) according to a previously described protocol 40
Second, it is able to induce the combination of ,-unsaturated ketones and GSH through the nucleophilic addition reaction, depleting GSH and reducing the amount of GSH available to combine with chemotherapeutic agents, thus producing an overall GST-inhibitory effect by sensitizing a cell to chemotherapeutic agents
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