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Materials and Methods GSH/GSSG-Glo Assay (Cat.# V6611) CellTox Green Cytotoxicity Assay (Cat.# G8741) menadione (Sigma, Cat.# M5625) l-Buthionine Sulfoximine (Sigma, Cat.# B2515) digitonin (Sigma, Cat.# D141) HEK-293 cells K-562 cells Note: Cell lines were authenticated using STR genotyping ( GenePrint 10 System, Cat.# B9510)

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- Detoxification: Glutathione supplements aid in liver detoxification, which removes toxins from the body and may benefit skin health

Additionally, glutathione regenerates other antioxidants like vitamins C and E, boosts immune function, and supports cellular repair and energy production