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Chronic fatigue suggests something in your protocol needs adjustment
Donaldson et al
Safety: What Are the Risks
After overnight culture, the cells were co-transfected with pGloSensor22F cAMP plasmid (Promega) and GLP-1R DNA using Lipofectamine 2000 transfection reagent (Invitrogen)
When finished, remove the needle and dispose of it in a proper sharps container
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