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However, an early report suggested that LECs rapidly change crystallin localization upon lens isolation (193), and now RNAseq experiments have shown that chicken (194), mouse (61, 195), rat (196), and human (160, 197, 198) LECs remodel between 10-33% of their transcriptome within a day of isolation including a robust upregulation of immediate early transcription factors and proinflammatory cytokine expression upon either isolation in culture (194), or following lens fiber cell removal surgery in vivo (61, 195)