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In the trial, 300 subjects were enrolled and presented with the Fragment 176-191 peptide for 12 weeks
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The beads were washed three times with washing buffer (20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM EDTA, 0.1% Triton X-100) via centrifugation at 500 g for 2 min at 4 C and once with 50 mM Tris-HCl, pH 7.5
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