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Vitamin b12 holds great safety profile and being a water soluble vitamin, it does not accumulate in body so no chance of toxicity is there
When cells reached ~3040% confluency (day 12), primitive streak (PS) induction was initiated using Advanced RPMI 1640 (Gibco, Thermo Fisher Scientific) supplemented with antibioticantimycotic (Gibco, #15240062), NEAAs, GlutaMax, B-27, and 8 M CHIR99021 (Tocris Bioscience) for 4 days
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"This is information we did not have before and it should be included in discussions between patients and their doctors, especially if patients have other known optic nerve problems like glaucoma or if there is preexisting significant visual loss from other causes," he added
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