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After washing with TBS containing 0.1% Tween 20, the membranes were incubated for 30 min at 25 C with TBS containing 0.1% Tween 20, 1% BSA, and horseradish peroxidase-conjugated anti-mouse (CST, 7076) or anti-rabbit (CST, 7074) secondary antibodies before visualization using Western Lightning ECL Pro (Perkin Elmer, NEL120001EA)
PMID: 25826983
Avoid repeated freeze-thaw cycles and keep materials in their original sealed container until ready for use
Vitamin B12 can be converted to coenzyme B12 in tissues
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