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Isolation and perfusion of the rat small and large intestine Non-fasted rats were anaesthetized with a subcutaneous injection of Hypnorm/Midazolam (0.0158 mg fentanyl citrate+0.5 mg fluanisone+0.25 mg midazolam/100 g)

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Transwell assay The chemotactic motility of HUVECs was determined using transwell migration chambers (Corning) with 6.5 mm diameter polycarbonate filters (8 m pore size), as described previously