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Carnitine and its derivatives were immediately determined in the supernatant using high-performance liquid chromatography (HPLC, VWR Hitachi, Radnor, Pennsylvania, USA) coupled with mass spectrometry (3200 Q Trap, AB Sciex, Framingham, Massachusetts, USA)

20 days old flies, Sp and/or L-DOPA fed from 10 days AEL, was used in all the experiments

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For example, NAD+ oral supplements are available, even though researchers say the molecule gets degraded in the gut and isn't absorbed well

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