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Activation triggers the PI3K-Akt-mTOR and Ras-MAPK pathways, promoting protein synthesis, glucose uptake, and cell proliferation
Shaking creates air bubbles and can cause physical damage to the peptide through shearing forces
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Wahyuni T et al
The reaction mixture was then concentrated with a 10k cut-off viva-spin 500 centrifugation tube, and washed twice with fresh PBS
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