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Counting and demultiplexing were performed using the BD Rhapsody Sequence Analysis Pipeline (v2.0), yielding around 10,000 called putative cells for each sample with a mean of around 45,000 aligned reads per cell
Furthermore, work in an cellspecific PC1/3KO mouse model demonstrates that loss of cellderived GLP-1 impairs the response to metabolic stress (20)
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