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5A-1MQ-treated mice switched from WD to LD (WD/LD-T) showed a rather reduced relative abundance of Akkermansia compared to vehicle-treated DIO animals switched from WD to LD (WD/LD-V), suggesting a unique microbiome mechanism associated with weight loss in the vehicle-treated group switched from WD to LD compared to the 5A-1MQ-treated group that underwent the same diet switch

The mean percentage of BrdU-labeled cells for three cultures per treatment was used as an indicator of activation (entry into the cell cycle) and the subsequent proliferation activity of plated satellite cells, according to Tatsumi et al

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