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The quantification of extracted DNA was performed using the Qubit DNA assay kit (Life Technologies, ThermoFisher Scientific, MA, USA)

Rotating injection sites and ensuring proper technique can help minimise them

MT-1 binds to melanocortin-1 receptors (MC1R) on melanocytes to stimulate eumelanin synthesis without the appetite suppression or sexual side effects associated with melanotan-2, making dose titration more straightforward

Reducing intake of sulfur-rich foods like eggs, dairy , and certain vegetables can drastically help

11 Other glucose-lowering treatments can be continued if needed for glycaemic control or cardiovascular or renal protection, with the dose of insulin or a sulfonylurea reduced to prevent hypoglycaemia, if required (see: Prescribing empagliflozin for guidance on dose reduction)