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As both H 2 O 2 and BCNU exposure led to excessive cellular GSSG accumulation, their impact on intracellular protein-GSSG adduct formation (protein S -glutathionylation) was subsequently examined in HepG2 cells by western blot analysis using a monoclonal GSSG antibody that specifically binds to GSSG-modified proteins

Finally, the sections were dehydrated and coverslipped using NEW M.X (Matsunami-glass)

This secondary dilution requires even more meticulous sterile technique and careful labeling to avoid confusion

Whether comparable propagation dynamics occur in three-dimensional tissues, where extracellular matrix constraints, variable cell density, heterogeneous antioxidant buffering, and local repair mechanisms are likely to shape spread, remains insufficiently resolved and presents a translational gap