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More importantly, first-pass metabolism in the liver reduces the fraction that reaches systemic circulation
RAW 264.7 macrophage cells were obtained from the Korean Cell Line Bank (Seoul, Korean) and cultured in DMEM containing 10% bovine calf serum (Gibco) and 1% penicillin/streptomycin (Gibco) at 37 C in an atmosphere of 95% humidified air/5% CO 2
Comment As in the case of hemangiomas, the occurrence of hemangiosarcomas in rodents has been well described in the published literature (Binhazim, Coghlan, and Walker 1994;Booth and Sundberg 1996
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