At specified time intervals (0 h, 1 h, 2 h, 4 h, 6 h, 8 h, 12 h, 24 h, 48 h), the sample solution (1.5 mL) was withdrawn from the dialysate, after which an equal volume of fresh corresponding solution (1.5 mL) was promptly added back into the dialysate to maintain the overall volume
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IV B12 protocols involve mixing the vitamin with saline and giving it over 30 minutes to an hour
These pooled samples were treated as a biological sample and analyzed amongst the analytical batches to assess instrument repeatability and potential batch effects in the data
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