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87 Notably, GSH protection against antibiotics did not require the AcrAB pump but failed in cells lacking the TolC channel, 88 suggesting that TolC-dependent pumps are involved

Consequently, NRF2 activity increases, leading to the elevated expression of antioxidant enzymes such as glutathione S-transferase, NAD(P)H quinone oxidoreductase 1, and heme oxygenase-1 (260)

The pathway may still be active, but may nonetheless not be necessary for the endpoint being investigated

These insights should be interpreted with a qualified healthcare provider who can contextualize results within your full clinical picture

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