After deparaffinization, sections were heated in an autoclave at 121 C for 5 min in 10 mM sodium citrate buffer (pH 6.0) or reacted with protease solution (Nichirei Biosciences, Tokyo, Japan) for epitope retrieval, after which endogenous peroxidase was inactivated by 0.3% hydrogen peroxide in methanol
Species can differ substantially in absorption, transport, hepatic metabolism, renal handling and off-target effects
PubMed, Reljic, Zorica, et al
Reconstitution should be performed by gently directing bacteriostatic water down the vial wall rather than directly onto the powder cake to prevent foaming and denaturation
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