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However, some of the widely used affinity tags, such as the His-tag, exhibit several drawbacks and limitations, since they can increase the risk of distorting the natural conformation of the target protein or necessitate stringent elution and wash conditions affecting the yield of the target protein

10.1096/fj.07-100735 114 YangQ.YamagataK.FukuiK.CaoY.NammoT.IwahashiH.et al (2002)

Yet, as we delve deeper, we find that microdosing raises important questions about clinical evidence, safety and whether a do-it-yourself approach truly pays off in the long run

Lola Thomas Post I've always been curious about how we can optimise the amount of energy in the body

Consistent use supports visible improvement over time