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According to the Canadian Society of Intestinal Research (CSIR), in the 1920s, a scientist suggested that coffee enemas would be more effective than saline enemas

Whole blood was collected from mice by cardiac puncture in BD vacutainer tubes (Fisher Scientific, Hampton, NH, USA) and centrifuged for 40 min at 2000 revolutions per minute to separate serum

Use the formula: Concentration after reconstitution = peptide mass divided by solvent volume For example, to get 0.2 mg/mL from a 1 mg vial, you would add 5 mL of solvent (1 mg 5 mL = 0.2 mg/mL)

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10.1371/journal.pone.0092900 PLoS One 74 ShriM.KumarS.ChakrabartyD.TrivediP