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The HPLC assay was conducted following a previously reported method by Liu Y et al., with a minor modification using an Agilent 1260 series system (USA) [30]
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Initially, orally administered vitamin B12 is tightly bound to the protein Haptocorrin (HC), encoded by the TCN1 gene, which is released into saliva to protect it from active, harmful factors, such as HCl, in the stomach and intestines [98, 99]
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