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However, upon counterselection, we consistently failed to recover a deletion mutant

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Eberl, D., Hegarty, B., Bossard, P., Ferr, P

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The staining solution was removed then and phenol red free L-15 medium were added before visualized by confocal microscopy (Zeiss LSM710, 63 NA 1.4 oil objective, using Ex 488 nm/Em 493582 nm (green) and Ex 488 nm/Em 599758 nm (red)

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