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Calibration samples were pipetted on spot cards, dried, and then used to quantify rapamycin in the unknown samples
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It is not constant and varies along with different factors, and the bill can differ from person to person
The binding affinity of purified anti-VEGF-scFv for VEGF were determined to be 25.8 pM, which was similar to that of developed anti-VEGF scFv drug (Brolucizumab, K d = 28.4 pM) 53
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