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A 2.5 mg dose would be 100 units (1 ml) on a standard U-100 insulin syringe, assuming a concentration of 2.5 mg per ml
Evidence across these actives is strongest in preclinical and early translational settings (for GHK-Cu, human cosmetic/dermatologic data also exist)
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This typically means 30-60 minutes before your first meal, upon waking
It does not demonstrate clinical efficacy, comprehensive safety, sterility, endotoxin control, or regulatory approval unless those attributes were specifically tested under suitable validated methods and the relevant regulatory requirements were met
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