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Cultures were rinsed in 10 mM phosphate buffered saline (PBS) and fixed with 4% paraformaldehyde (PFA) in phosphate buffer (PB)

Overall, across the two repeat experiments for Cagri and CagriBB bound to the CTR, the conformational equilibrium between the bypass vs CT-like state was similar, suggesting that the salt bridge/ionic lock is sufficient to preferentially stabilize the bypass conformation at CTR (Supplementary Fig

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It not only serves as a major risk factor for chronic diseases such as cardiovascular disease and diabetes but also exacerbates these conditions, further complicating their management and outcomes

Disease burden and economic impact of diagnosed non-alcoholic steatohepatitis in five European countries in 2018: a cost-of-illness analysis