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glabrata atg1 strain was constructed using a one-step PCR-based technique as described previously ( CgATG1 deletion construct was amplified from pBSK-HIS using primers tagged with 100-bp sequences homologous to the flanking regions of the CgATG1 ORF (CgATG1-100F and CgATG1-100R)

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Interestingly, some metabolites such as malate, -ketoglutarate, ornithine, glutamate, ornithine, phenylalanine, tryptophan, tyrosine, leucine, and histidine had higher percentage 13 C-labelling in three or more carbon atoms (M+ 3) in the gdh3 / gdh3 mutant compared to wild-type cells grown in 30% U- 13 C 5 proline medium