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In vitro material was kindly provided by the Julius Khn-Insitute (JKI), Federal Research Centre for Cultivated Plants Institute for Resistance Research and Stress Tolerance, Gro Lsewitz, and cultivated in 500 ml polypropylene (PP) vessels (Plastikbecher.de GmbH, Giengen, DE) on 80 ml solid MS (Murashige and Skoog 1962) medium (3% (w/v) sucrose, 7.5 g/l Plant agar (Duchefa Biochemie B.V., Haarlem, The Netherlands), pH 5.8) at 18 2 C in a long day photoperiod (16 h light/8 h dark) with a photon flux density of ~ 35 8 mol m 2 s 1
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