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After 24 h of seeding, drugs or vehicle controls were added, and live cell imaging was performed using the IncuCyte S3 system (ESSEN BioSciences, Ann Arbor, MI, USA) at 10x magnification every 6 h over a period of 96 h (2 images per well per timepoint, 17 total timepoints)

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Tirzepatide : Approved in 2022 (Mounjaro) and 2024 (Zepbound), its NCE exclusivity runs to May 2027, delaying generic filings until 2026