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When you insert the needle through the vial stopper and invert the vial, the needle bevel (the angled opening at the tip) should face upward, away from the bottom of the vial

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Deep sequencing using Sentosa SQ HCV Genotyping Assay Briefly, nucleic acid extraction was performed from 530 L of serum on the Sentosa SX101 robotic instrument using Sentosa Virus Total Nucleic Acid Plus II kit