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Add the clear sample containing target GST-tagged protein onto the equilibrated gravity column, keep the sample in column for at least 2 min, ensure that the sample fully binds with the resin, and collect the flowthrough with a flow-rate of 0.5 - 1 mL per minute, then add the flowthrough onto the column again, repeat the previous operation, this can increase the binding efficiency

Polyphenols-classified divided into flavonoid and non-flavonoid groups are abundant in fruits and plants

A competition immunoprecipitation assay of unlabeled poliovirus antigens

However, symptoms are far more likely to be related to underlying conditions that cause B12 elevationsuch as liver disease, kidney dysfunction, or haematological disordersrather than the elevated B12 itself