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Previous work has shown that the abundance of Lactobacillus reuteri (normalized to a control group) decreases during the onset of obesity and remains suppressed even after dieting in mice 99 , which parallels our observations that vehicle-treated diet-switched mice had lower levels of Lactobacillus than the 5A-1MQ-treated mice, the latter of which also demonstrated greater weight loss

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While treatment-induced changes in liver metabolic markers, inflammation, and endoplasmic reticulum stress were largely explained by weight loss, a subset of ECM-related genes remained suppressed after adjusting for body weight in DIO-MASH mice