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Identity confirmation is typically performed using liquid chromatographymass spectrometry (LC-MS), ensuring molecular consistency with the intended peptide sequence

Finally, the plate was scanned on the Odyssey Infrared Imager and the integrated intensities of fluorescence were determined by the LI-COR Image Studio software

Thus, while acute LC supplementation may confer immediate physiological benefits, chronic elevations in TMAO levels warrant careful risk evaluation due to their association with adverse cardiovascular outcomes, particularly in vulnerable populations

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