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The obtained homogenates were then mixed with the same volumes of 10% (v/v) TCA-4% (v/v) 2-mercaptoethanol and allowed to stand for 30 min at 25C

After TMB substrate solution is added, only those wells that contain Rat IFNg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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Our findings demonstrated that Fer-1 reversed the cell death caused by Irinotecan and Oxaliplatin treatment in SW480 and HCT116 cells (Fig

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