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The qPCR cycling conditions were as follows: an initial denaturation at 95 C for 15 min, followed by 30 cycles of 95 C for 7 s and 58 C for 10 s, and a final extension step at 95 C for 5 min, then 40 cycles of 95 C for 15 s and 58 C for 30 s

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What You Will Learn Module 1: Scientific Foundations of NAD The biochemistry of NAD Cellular metabolism & mitochondrial function NAD and ageing pathways DNA repair and oxidative stress Clinical rationale for supplementation Module 2: Evidence-Based Treatment Protocols NAD IV infusion protocols Glutathione integration strategies Adjunctive NAD support (IV, IM, oral) Contraindications and patient screening Protocol structuring and dosage rationale Module 3: IV Cannulation Principles (Theory-Based) Suitable venous access sites Cannula selection & preparation Step-by-step theoretical technique overview Monitoring during infusion Risk awareness and complication management Module 4: Professional Standards & Clinical Governance Prescriber workflow Scope of practice clarity Legal and regulatory considerations Pricing Strategies Additional Materials Included Unbranded Consent Form Template Independent Prescriber Email Template Patient Specific Direction Template Prescriber Authorisation Request Form IV Observation Chart All materials are provided digitally for self-paced study

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