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AQP4 deficiency in mice leads to severe neuroinflammation, increased microglial activity, and neuronal damage [61]

Western blot analysis and immunoprecipitation Total cells lysates for immunoblotting or immunoprecipitation were lysed with buffer (0.1%Triton-100, 50 mM Tris-Cl, pH 7.5, 0.1% NP-40, 150 mM NaCl, 0.1 M EDTA) supplemented with a cocktail of phosphate and proteinase inhibitors for 30 min at 4 C

Step 7: Dispose of the used syringe in your sharps container

It is naturally present in human blood, saliva, urine, and its plasma concentration declines with age from approximately 200 ng/mL at age 20 to under 80 ng/mL after age 60

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