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After the washing step the R6G dye of the internalized non-targeted or targeted nanocarriers in living hECs was imaged using the 543 nm laser line on a Leica TCS SP5 confocal laser scanning microscope equipped with a sample holder kept at 37 C
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Reduced calorie diet and increased physical activity, to reduce the risk of major adverse cardiovascular events (cardiovascular death, non-fatal myocardial infarction, or non-fatal stroke) in adults with established cardiovascular disease and who are either obese or overweight
