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Similar approaches have successfully generated cardiac spheroids using rat neonatal ventricular cardiomyocytes, human dermal fibroblasts, and human coronary microartery ECs in ultra-low attachment plates [253], as well as spheroids composed of human iPSC-derived cardiomyocytes, human cardiac fibroblasts, and HUVECs in an ultra-low attachment hanging drop system [254]
Absorbance was measured at 490 nm using a microplate reader (Epoch, Bio Teck, Winooski, VT, USA)
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Both use the same 5-days-on/2-days-off schedule, making them naturally synchronized
This could reflect unresolved damage from the anterior cruciate ligament injury surgery used in this study, although there is also the possibility that removal of senescent cells without targeting the causes that induce their accumulation could limit the benefits of senolytics
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