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Immunoprecipitation was then performed using the Catch and Release Immunoprecipitation Kit (EMD Millipore), according to the manufacturers instructions with 250 g of total protein from cells or 400 g from tissues, and 1 g of an anti-IGF-1R antibody (#9750, Cell Signaling)

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Bracci M, Ciarapica V, Copertaro A, Barbaresi M, Manzella N, Tomasetti M, Gaetani S, Monaco F, Amati M, Valentino M, Rapisarda V, Santarelli L (2016) Peripheral skin temperature and circadian biological clock in shift nurses after a day off

Parrish AB, Freel CD, Kornbluth S