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doi: 10.1016/S0166-445X(01)00236-3 341 Wilkes WalburnJ.WemheuerB.ThomasT.CopelandE.OConnorW.BoothM.et al
Briefly, 25-30 million cells were collected and washed twice with cold saline (0.9% NaCl), scraped into 1 ml of cold KPBS, and pelleted via centrifugation at 1,000g for 1.5min at 4C
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Tuy nhin, iu quan trng l hiu ng v l trnh s dng, bit khi no nn tn cng v khi no nn chuyn sang duy tr, ng thi la chn dng bo ch ph hp ti u hiu qu hp thu
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