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The compound distributes preferentially to metabolically active organs, showing highest concentrations in the kidneys and liver, followed by the stomach wall, thymus, spleen, and intestines
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While not dangerous, they indicate significant vascular reactivity and suggest the nitric oxide system is responding robustly to the peptide
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A structural explanation for these findings was provided from our previous cryo-EM structures of CT(Pro32) and rAmy(Tyr37)-bound AMYRs structures where the bulky phenolic side chain of Y37 rAmy forms perpendicular stacking interactions with W79 ECD 15 , and additional interactions with the respective RAMP protomer that are likely important for selectivity of rAmy for AMYRs over CTR
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