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In-vitro models and readouts GHS-R1a couples principally to Gq, so inositol phosphate accumulation and intracellular calcium mobilization are the direct readouts, with recombinant lines expressing human GHS-R1a as the usual system
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Clarke, J
The fact that the double-cysteine mutations only partially abolish the physiological function may imply that not all the expressed mutants have an intact disulfide bridge and that the observed conformation is only one possible inactive state on the cell surface, as supported by the EM data and MD simulations below
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