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Common cellular and molecular mechanisms in obesity and drug addiction

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CPA digestion of albumin To initiate in vitro enzymatic cleavage by CPA, unfused albumin (WT or QMP) or albumin fused to GLP-1, FIX 9 , or ACE2 13 (15 M), in DPBS (pH 7.4) (Sigma-Aldrich), were incubated with 30 g/mL of human pancreatic CPA (Elastin Products Company, Inc.) for 5 h at 37 C on a shaker platform set to 450 RPM

The mechanistic explanation: GLP-1 receptors are expressed in brain regions involved in reward processing specifically the ventral tegmental area (VTA) and nucleus accumbens (NAc), both central to the reinforcing effects of alcohol, food, and other substances

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