Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step
Kocsis K, Frank R, Szab J, Knapp L, Kis Z, Farkas T, Vcsei L, Toldi J (2016) Acetyl-l-carnitine restores synaptic transmission and enhances the inducibility of stable LTP after oxygen-glucose deprivation
The extracted sample is divided on four parts for selective measurement of the metabolites (Fig
The mixture was diluted with deionized water (200 mL) and filtered
For the negative controls, the identical procedure was followed, with the exception that PBS was used instead of the primary antibody